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lipolysis bmp7 mouse 5666 bp 010 r d systems flow cytometry bmp9 human 3209 bp 010 r d systems flow cytometry  (R&D Systems)


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    R&D Systems lipolysis bmp7 mouse 5666 bp 010 r d systems flow cytometry bmp9 human 3209 bp 010 r d systems flow cytometry
    Lipolysis Bmp7 Mouse 5666 Bp 010 R D Systems Flow Cytometry Bmp9 Human 3209 Bp 010 R D Systems Flow Cytometry, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+bmp7+r+d+systems/Recombinant+Mouse+BMP-7+Protein/pmc12075709__41467_2025_59673_MOESM1_ESM-11-57-61
    Average 94 stars, based on 27 article reviews
    lipolysis bmp7 mouse 5666 bp 010 r d systems flow cytometry bmp9 human 3209 bp 010 r d systems flow cytometry - by Bioz Stars, 2026-10
    94/100 stars

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    Recombinant:

    Article Title: ActRII or BMPR ligands inhibit skeletal myoblast differentiation, and BMPs promote heterotopic ossification in skeletal muscles in mice
    Article Snippet: Rabbit monoclonal anti-phospho-SMAD2 (Ser465/467) Cell Signaling Technology Cat#3108; RRID:AB_490941 Rabbit monoclonal phospho-Smad3 (Ser423/425) Cell Signaling Technology Cat#9520; RRID:AB_2193207 Rabbit monoclonal anti-SMAD2/3 Cell Signaling Technology Cat#8685; RRID:AB_10889933 Rabbit monoclonal anti-SMAD3 Cell Signaling Technology Cat#9523; RRID:AB_2193182 Rabbit monoclonal phospho-Smad1 (Ser206) Cell Signaling Technology Cat#5753; RRID:AB_10706171 Rabbit monoclonal phospho-Smad1/5 (Ser463/465) Cell Signaling Technology Cat#9516; RRID:AB_491015 Rabbit polyclonal anti-SMAD1 Cell Signaling Technology Cat#9743; RRID:AB_2107780 Rabbit polyclonal anti-SMAD5 Cell Signaling Technology Cat#9517; RRID:AB_2193632 Rabbit monoclonal anti-vinculin Cell Signaling Technology Cat#13901; RRID:AB_2728768 Rabbit monoclonal anti-GAPDH Sigma Aldrich Cat#G9295-200ul Peroxidase AffiniPureTM Goat Anti-Rabbit IgG Jackson Cat#111-035-003 Mouse monoclonal anti-Myosin Heavy Chain/MYHC antibody [clone A4.1025] Abcam Cat#ab37484; RRID:AB_2921304 Goat polyclonal anti-Mouse IgG, Alexa FluorTM Plus 488 Thermo Fisher Scientific Cat#A32723; RRID:AB_2633275 .. PBS Gibco Cat#14190-144 BSA Sigma Aldrich Cat#A0336 Glycerol invitrogen Cat#15514011 Recombinant human/mouse/rat Activin A R&D Systems Cat#338-AC Recombinant human/mouse/rat GDF8 R&D Systems Cat#788-G8 Recombinant human/mouse/rat GDF11 R&D Systems Cat#1958-GD Recombinant human BMP2 R&D Systems Cat#355-BM Recombinant human BMP4 R&D Systems Cat#314-BP Recombinant human BMP5 R&D Systems Cat#615-BMC Recombinant human BMP6 R&D Systems Cat#507-BP Recombinant human BMP7 R&D Systems Cat354-BP Recombinant human BMP9 R&D Systems Cat#3209-BP Recombinant human BMP10 R&D Systems Cat#2926-BP Recombinant mouse BMP13 R&D Systems Cat#855-G6 Regeneron - Internal Recombinant human BMP14 R&D Systems Cat#8340-G5 Recombinant human long-R3-IGF1 R&D System Cat#83350-GMP Recombinant human TNFa R&D Systems Cat#210-TA/CF Anti-ActRIIA/B monoclonal antibody Regeneron N/A MyoTonic Basal Medium Cook MyoSite Cat#MB-2222 MyoTonic Growth Supplement Cook MyoSite Cat#MS-3333 Serum-free MyoTonic differentiation medium Cook MyoSite Cat#MD-9999 Horse serum Gibco Cat#16050130 Penicillin-Streptomycin (10,000 U/mL) Gibco Cat#15140-122 0.025% Trypsin Gibco Cat#25300-054 Puromycin Gibco Cat#A11138-03 Goat serum Thermo Fisher Scientific Cat#500622 Triton-X 100 Sigma Aldrich Cat#93443 DAPI Biolegend Cat#422801 RIPA Lysis Buffer Thermo Fisher Scientific Cat#89900 HaltTM protease and phosphatase inhibitor Thermo Fisher Scientific Cat#78440 Immobilon® Block Chemiluminescent Blocker Millipore Cat#WBAVDCH01 PierceTM ECL Western Blotting Substrate Thermo Fisher Scientific Cat#32106 Immobilon Chemiluminescent HRP Substrate Millipore Cat#WBKLS0500 TBS with TweenTM (TBST) Thermo Fisher Scientific Cat#J77500-K8 Commercial Assays PierceTM BCA Protein Assay Kits Thermo Fisher Scientific Cat#23227 MagMax-96 total RNA isolation kit Thermo Fisher Scientific Cat#AM1830 SuperScript II VILO cDNA Synthesis kit Thermo Fisher Scientific Cat#11754250 .. Human Skeletal Muscle Derived Cells (SKMDC) Cook Myosite Cat#SK-1111; Lot# P01059-14M

    Lysis:

    Article Title: ActRII or BMPR ligands inhibit skeletal myoblast differentiation, and BMPs promote heterotopic ossification in skeletal muscles in mice
    Article Snippet: Rabbit monoclonal anti-phospho-SMAD2 (Ser465/467) Cell Signaling Technology Cat#3108; RRID:AB_490941 Rabbit monoclonal phospho-Smad3 (Ser423/425) Cell Signaling Technology Cat#9520; RRID:AB_2193207 Rabbit monoclonal anti-SMAD2/3 Cell Signaling Technology Cat#8685; RRID:AB_10889933 Rabbit monoclonal anti-SMAD3 Cell Signaling Technology Cat#9523; RRID:AB_2193182 Rabbit monoclonal phospho-Smad1 (Ser206) Cell Signaling Technology Cat#5753; RRID:AB_10706171 Rabbit monoclonal phospho-Smad1/5 (Ser463/465) Cell Signaling Technology Cat#9516; RRID:AB_491015 Rabbit polyclonal anti-SMAD1 Cell Signaling Technology Cat#9743; RRID:AB_2107780 Rabbit polyclonal anti-SMAD5 Cell Signaling Technology Cat#9517; RRID:AB_2193632 Rabbit monoclonal anti-vinculin Cell Signaling Technology Cat#13901; RRID:AB_2728768 Rabbit monoclonal anti-GAPDH Sigma Aldrich Cat#G9295-200ul Peroxidase AffiniPureTM Goat Anti-Rabbit IgG Jackson Cat#111-035-003 Mouse monoclonal anti-Myosin Heavy Chain/MYHC antibody [clone A4.1025] Abcam Cat#ab37484; RRID:AB_2921304 Goat polyclonal anti-Mouse IgG, Alexa FluorTM Plus 488 Thermo Fisher Scientific Cat#A32723; RRID:AB_2633275 .. PBS Gibco Cat#14190-144 BSA Sigma Aldrich Cat#A0336 Glycerol invitrogen Cat#15514011 Recombinant human/mouse/rat Activin A R&D Systems Cat#338-AC Recombinant human/mouse/rat GDF8 R&D Systems Cat#788-G8 Recombinant human/mouse/rat GDF11 R&D Systems Cat#1958-GD Recombinant human BMP2 R&D Systems Cat#355-BM Recombinant human BMP4 R&D Systems Cat#314-BP Recombinant human BMP5 R&D Systems Cat#615-BMC Recombinant human BMP6 R&D Systems Cat#507-BP Recombinant human BMP7 R&D Systems Cat354-BP Recombinant human BMP9 R&D Systems Cat#3209-BP Recombinant human BMP10 R&D Systems Cat#2926-BP Recombinant mouse BMP13 R&D Systems Cat#855-G6 Regeneron - Internal Recombinant human BMP14 R&D Systems Cat#8340-G5 Recombinant human long-R3-IGF1 R&D System Cat#83350-GMP Recombinant human TNFa R&D Systems Cat#210-TA/CF Anti-ActRIIA/B monoclonal antibody Regeneron N/A MyoTonic Basal Medium Cook MyoSite Cat#MB-2222 MyoTonic Growth Supplement Cook MyoSite Cat#MS-3333 Serum-free MyoTonic differentiation medium Cook MyoSite Cat#MD-9999 Horse serum Gibco Cat#16050130 Penicillin-Streptomycin (10,000 U/mL) Gibco Cat#15140-122 0.025% Trypsin Gibco Cat#25300-054 Puromycin Gibco Cat#A11138-03 Goat serum Thermo Fisher Scientific Cat#500622 Triton-X 100 Sigma Aldrich Cat#93443 DAPI Biolegend Cat#422801 RIPA Lysis Buffer Thermo Fisher Scientific Cat#89900 HaltTM protease and phosphatase inhibitor Thermo Fisher Scientific Cat#78440 Immobilon® Block Chemiluminescent Blocker Millipore Cat#WBAVDCH01 PierceTM ECL Western Blotting Substrate Thermo Fisher Scientific Cat#32106 Immobilon Chemiluminescent HRP Substrate Millipore Cat#WBKLS0500 TBS with TweenTM (TBST) Thermo Fisher Scientific Cat#J77500-K8 Commercial Assays PierceTM BCA Protein Assay Kits Thermo Fisher Scientific Cat#23227 MagMax-96 total RNA isolation kit Thermo Fisher Scientific Cat#AM1830 SuperScript II VILO cDNA Synthesis kit Thermo Fisher Scientific Cat#11754250 .. Human Skeletal Muscle Derived Cells (SKMDC) Cook Myosite Cat#SK-1111; Lot# P01059-14M

    Blocking Assay:

    Article Title: ActRII or BMPR ligands inhibit skeletal myoblast differentiation, and BMPs promote heterotopic ossification in skeletal muscles in mice
    Article Snippet: Rabbit monoclonal anti-phospho-SMAD2 (Ser465/467) Cell Signaling Technology Cat#3108; RRID:AB_490941 Rabbit monoclonal phospho-Smad3 (Ser423/425) Cell Signaling Technology Cat#9520; RRID:AB_2193207 Rabbit monoclonal anti-SMAD2/3 Cell Signaling Technology Cat#8685; RRID:AB_10889933 Rabbit monoclonal anti-SMAD3 Cell Signaling Technology Cat#9523; RRID:AB_2193182 Rabbit monoclonal phospho-Smad1 (Ser206) Cell Signaling Technology Cat#5753; RRID:AB_10706171 Rabbit monoclonal phospho-Smad1/5 (Ser463/465) Cell Signaling Technology Cat#9516; RRID:AB_491015 Rabbit polyclonal anti-SMAD1 Cell Signaling Technology Cat#9743; RRID:AB_2107780 Rabbit polyclonal anti-SMAD5 Cell Signaling Technology Cat#9517; RRID:AB_2193632 Rabbit monoclonal anti-vinculin Cell Signaling Technology Cat#13901; RRID:AB_2728768 Rabbit monoclonal anti-GAPDH Sigma Aldrich Cat#G9295-200ul Peroxidase AffiniPureTM Goat Anti-Rabbit IgG Jackson Cat#111-035-003 Mouse monoclonal anti-Myosin Heavy Chain/MYHC antibody [clone A4.1025] Abcam Cat#ab37484; RRID:AB_2921304 Goat polyclonal anti-Mouse IgG, Alexa FluorTM Plus 488 Thermo Fisher Scientific Cat#A32723; RRID:AB_2633275 .. PBS Gibco Cat#14190-144 BSA Sigma Aldrich Cat#A0336 Glycerol invitrogen Cat#15514011 Recombinant human/mouse/rat Activin A R&D Systems Cat#338-AC Recombinant human/mouse/rat GDF8 R&D Systems Cat#788-G8 Recombinant human/mouse/rat GDF11 R&D Systems Cat#1958-GD Recombinant human BMP2 R&D Systems Cat#355-BM Recombinant human BMP4 R&D Systems Cat#314-BP Recombinant human BMP5 R&D Systems Cat#615-BMC Recombinant human BMP6 R&D Systems Cat#507-BP Recombinant human BMP7 R&D Systems Cat354-BP Recombinant human BMP9 R&D Systems Cat#3209-BP Recombinant human BMP10 R&D Systems Cat#2926-BP Recombinant mouse BMP13 R&D Systems Cat#855-G6 Regeneron - Internal Recombinant human BMP14 R&D Systems Cat#8340-G5 Recombinant human long-R3-IGF1 R&D System Cat#83350-GMP Recombinant human TNFa R&D Systems Cat#210-TA/CF Anti-ActRIIA/B monoclonal antibody Regeneron N/A MyoTonic Basal Medium Cook MyoSite Cat#MB-2222 MyoTonic Growth Supplement Cook MyoSite Cat#MS-3333 Serum-free MyoTonic differentiation medium Cook MyoSite Cat#MD-9999 Horse serum Gibco Cat#16050130 Penicillin-Streptomycin (10,000 U/mL) Gibco Cat#15140-122 0.025% Trypsin Gibco Cat#25300-054 Puromycin Gibco Cat#A11138-03 Goat serum Thermo Fisher Scientific Cat#500622 Triton-X 100 Sigma Aldrich Cat#93443 DAPI Biolegend Cat#422801 RIPA Lysis Buffer Thermo Fisher Scientific Cat#89900 HaltTM protease and phosphatase inhibitor Thermo Fisher Scientific Cat#78440 Immobilon® Block Chemiluminescent Blocker Millipore Cat#WBAVDCH01 PierceTM ECL Western Blotting Substrate Thermo Fisher Scientific Cat#32106 Immobilon Chemiluminescent HRP Substrate Millipore Cat#WBKLS0500 TBS with TweenTM (TBST) Thermo Fisher Scientific Cat#J77500-K8 Commercial Assays PierceTM BCA Protein Assay Kits Thermo Fisher Scientific Cat#23227 MagMax-96 total RNA isolation kit Thermo Fisher Scientific Cat#AM1830 SuperScript II VILO cDNA Synthesis kit Thermo Fisher Scientific Cat#11754250 .. Human Skeletal Muscle Derived Cells (SKMDC) Cook Myosite Cat#SK-1111; Lot# P01059-14M

    Western Blot:

    Article Title: ActRII or BMPR ligands inhibit skeletal myoblast differentiation, and BMPs promote heterotopic ossification in skeletal muscles in mice
    Article Snippet: Rabbit monoclonal anti-phospho-SMAD2 (Ser465/467) Cell Signaling Technology Cat#3108; RRID:AB_490941 Rabbit monoclonal phospho-Smad3 (Ser423/425) Cell Signaling Technology Cat#9520; RRID:AB_2193207 Rabbit monoclonal anti-SMAD2/3 Cell Signaling Technology Cat#8685; RRID:AB_10889933 Rabbit monoclonal anti-SMAD3 Cell Signaling Technology Cat#9523; RRID:AB_2193182 Rabbit monoclonal phospho-Smad1 (Ser206) Cell Signaling Technology Cat#5753; RRID:AB_10706171 Rabbit monoclonal phospho-Smad1/5 (Ser463/465) Cell Signaling Technology Cat#9516; RRID:AB_491015 Rabbit polyclonal anti-SMAD1 Cell Signaling Technology Cat#9743; RRID:AB_2107780 Rabbit polyclonal anti-SMAD5 Cell Signaling Technology Cat#9517; RRID:AB_2193632 Rabbit monoclonal anti-vinculin Cell Signaling Technology Cat#13901; RRID:AB_2728768 Rabbit monoclonal anti-GAPDH Sigma Aldrich Cat#G9295-200ul Peroxidase AffiniPureTM Goat Anti-Rabbit IgG Jackson Cat#111-035-003 Mouse monoclonal anti-Myosin Heavy Chain/MYHC antibody [clone A4.1025] Abcam Cat#ab37484; RRID:AB_2921304 Goat polyclonal anti-Mouse IgG, Alexa FluorTM Plus 488 Thermo Fisher Scientific Cat#A32723; RRID:AB_2633275 .. PBS Gibco Cat#14190-144 BSA Sigma Aldrich Cat#A0336 Glycerol invitrogen Cat#15514011 Recombinant human/mouse/rat Activin A R&D Systems Cat#338-AC Recombinant human/mouse/rat GDF8 R&D Systems Cat#788-G8 Recombinant human/mouse/rat GDF11 R&D Systems Cat#1958-GD Recombinant human BMP2 R&D Systems Cat#355-BM Recombinant human BMP4 R&D Systems Cat#314-BP Recombinant human BMP5 R&D Systems Cat#615-BMC Recombinant human BMP6 R&D Systems Cat#507-BP Recombinant human BMP7 R&D Systems Cat354-BP Recombinant human BMP9 R&D Systems Cat#3209-BP Recombinant human BMP10 R&D Systems Cat#2926-BP Recombinant mouse BMP13 R&D Systems Cat#855-G6 Regeneron - Internal Recombinant human BMP14 R&D Systems Cat#8340-G5 Recombinant human long-R3-IGF1 R&D System Cat#83350-GMP Recombinant human TNFa R&D Systems Cat#210-TA/CF Anti-ActRIIA/B monoclonal antibody Regeneron N/A MyoTonic Basal Medium Cook MyoSite Cat#MB-2222 MyoTonic Growth Supplement Cook MyoSite Cat#MS-3333 Serum-free MyoTonic differentiation medium Cook MyoSite Cat#MD-9999 Horse serum Gibco Cat#16050130 Penicillin-Streptomycin (10,000 U/mL) Gibco Cat#15140-122 0.025% Trypsin Gibco Cat#25300-054 Puromycin Gibco Cat#A11138-03 Goat serum Thermo Fisher Scientific Cat#500622 Triton-X 100 Sigma Aldrich Cat#93443 DAPI Biolegend Cat#422801 RIPA Lysis Buffer Thermo Fisher Scientific Cat#89900 HaltTM protease and phosphatase inhibitor Thermo Fisher Scientific Cat#78440 Immobilon® Block Chemiluminescent Blocker Millipore Cat#WBAVDCH01 PierceTM ECL Western Blotting Substrate Thermo Fisher Scientific Cat#32106 Immobilon Chemiluminescent HRP Substrate Millipore Cat#WBKLS0500 TBS with TweenTM (TBST) Thermo Fisher Scientific Cat#J77500-K8 Commercial Assays PierceTM BCA Protein Assay Kits Thermo Fisher Scientific Cat#23227 MagMax-96 total RNA isolation kit Thermo Fisher Scientific Cat#AM1830 SuperScript II VILO cDNA Synthesis kit Thermo Fisher Scientific Cat#11754250 .. Human Skeletal Muscle Derived Cells (SKMDC) Cook Myosite Cat#SK-1111; Lot# P01059-14M

    Bicinchoninic Acid Protein Assay:

    Article Title: ActRII or BMPR ligands inhibit skeletal myoblast differentiation, and BMPs promote heterotopic ossification in skeletal muscles in mice
    Article Snippet: Rabbit monoclonal anti-phospho-SMAD2 (Ser465/467) Cell Signaling Technology Cat#3108; RRID:AB_490941 Rabbit monoclonal phospho-Smad3 (Ser423/425) Cell Signaling Technology Cat#9520; RRID:AB_2193207 Rabbit monoclonal anti-SMAD2/3 Cell Signaling Technology Cat#8685; RRID:AB_10889933 Rabbit monoclonal anti-SMAD3 Cell Signaling Technology Cat#9523; RRID:AB_2193182 Rabbit monoclonal phospho-Smad1 (Ser206) Cell Signaling Technology Cat#5753; RRID:AB_10706171 Rabbit monoclonal phospho-Smad1/5 (Ser463/465) Cell Signaling Technology Cat#9516; RRID:AB_491015 Rabbit polyclonal anti-SMAD1 Cell Signaling Technology Cat#9743; RRID:AB_2107780 Rabbit polyclonal anti-SMAD5 Cell Signaling Technology Cat#9517; RRID:AB_2193632 Rabbit monoclonal anti-vinculin Cell Signaling Technology Cat#13901; RRID:AB_2728768 Rabbit monoclonal anti-GAPDH Sigma Aldrich Cat#G9295-200ul Peroxidase AffiniPureTM Goat Anti-Rabbit IgG Jackson Cat#111-035-003 Mouse monoclonal anti-Myosin Heavy Chain/MYHC antibody [clone A4.1025] Abcam Cat#ab37484; RRID:AB_2921304 Goat polyclonal anti-Mouse IgG, Alexa FluorTM Plus 488 Thermo Fisher Scientific Cat#A32723; RRID:AB_2633275 .. PBS Gibco Cat#14190-144 BSA Sigma Aldrich Cat#A0336 Glycerol invitrogen Cat#15514011 Recombinant human/mouse/rat Activin A R&D Systems Cat#338-AC Recombinant human/mouse/rat GDF8 R&D Systems Cat#788-G8 Recombinant human/mouse/rat GDF11 R&D Systems Cat#1958-GD Recombinant human BMP2 R&D Systems Cat#355-BM Recombinant human BMP4 R&D Systems Cat#314-BP Recombinant human BMP5 R&D Systems Cat#615-BMC Recombinant human BMP6 R&D Systems Cat#507-BP Recombinant human BMP7 R&D Systems Cat354-BP Recombinant human BMP9 R&D Systems Cat#3209-BP Recombinant human BMP10 R&D Systems Cat#2926-BP Recombinant mouse BMP13 R&D Systems Cat#855-G6 Regeneron - Internal Recombinant human BMP14 R&D Systems Cat#8340-G5 Recombinant human long-R3-IGF1 R&D System Cat#83350-GMP Recombinant human TNFa R&D Systems Cat#210-TA/CF Anti-ActRIIA/B monoclonal antibody Regeneron N/A MyoTonic Basal Medium Cook MyoSite Cat#MB-2222 MyoTonic Growth Supplement Cook MyoSite Cat#MS-3333 Serum-free MyoTonic differentiation medium Cook MyoSite Cat#MD-9999 Horse serum Gibco Cat#16050130 Penicillin-Streptomycin (10,000 U/mL) Gibco Cat#15140-122 0.025% Trypsin Gibco Cat#25300-054 Puromycin Gibco Cat#A11138-03 Goat serum Thermo Fisher Scientific Cat#500622 Triton-X 100 Sigma Aldrich Cat#93443 DAPI Biolegend Cat#422801 RIPA Lysis Buffer Thermo Fisher Scientific Cat#89900 HaltTM protease and phosphatase inhibitor Thermo Fisher Scientific Cat#78440 Immobilon® Block Chemiluminescent Blocker Millipore Cat#WBAVDCH01 PierceTM ECL Western Blotting Substrate Thermo Fisher Scientific Cat#32106 Immobilon Chemiluminescent HRP Substrate Millipore Cat#WBKLS0500 TBS with TweenTM (TBST) Thermo Fisher Scientific Cat#J77500-K8 Commercial Assays PierceTM BCA Protein Assay Kits Thermo Fisher Scientific Cat#23227 MagMax-96 total RNA isolation kit Thermo Fisher Scientific Cat#AM1830 SuperScript II VILO cDNA Synthesis kit Thermo Fisher Scientific Cat#11754250 .. Human Skeletal Muscle Derived Cells (SKMDC) Cook Myosite Cat#SK-1111; Lot# P01059-14M

    Isolation:

    Article Title: ActRII or BMPR ligands inhibit skeletal myoblast differentiation, and BMPs promote heterotopic ossification in skeletal muscles in mice
    Article Snippet: Rabbit monoclonal anti-phospho-SMAD2 (Ser465/467) Cell Signaling Technology Cat#3108; RRID:AB_490941 Rabbit monoclonal phospho-Smad3 (Ser423/425) Cell Signaling Technology Cat#9520; RRID:AB_2193207 Rabbit monoclonal anti-SMAD2/3 Cell Signaling Technology Cat#8685; RRID:AB_10889933 Rabbit monoclonal anti-SMAD3 Cell Signaling Technology Cat#9523; RRID:AB_2193182 Rabbit monoclonal phospho-Smad1 (Ser206) Cell Signaling Technology Cat#5753; RRID:AB_10706171 Rabbit monoclonal phospho-Smad1/5 (Ser463/465) Cell Signaling Technology Cat#9516; RRID:AB_491015 Rabbit polyclonal anti-SMAD1 Cell Signaling Technology Cat#9743; RRID:AB_2107780 Rabbit polyclonal anti-SMAD5 Cell Signaling Technology Cat#9517; RRID:AB_2193632 Rabbit monoclonal anti-vinculin Cell Signaling Technology Cat#13901; RRID:AB_2728768 Rabbit monoclonal anti-GAPDH Sigma Aldrich Cat#G9295-200ul Peroxidase AffiniPureTM Goat Anti-Rabbit IgG Jackson Cat#111-035-003 Mouse monoclonal anti-Myosin Heavy Chain/MYHC antibody [clone A4.1025] Abcam Cat#ab37484; RRID:AB_2921304 Goat polyclonal anti-Mouse IgG, Alexa FluorTM Plus 488 Thermo Fisher Scientific Cat#A32723; RRID:AB_2633275 .. PBS Gibco Cat#14190-144 BSA Sigma Aldrich Cat#A0336 Glycerol invitrogen Cat#15514011 Recombinant human/mouse/rat Activin A R&D Systems Cat#338-AC Recombinant human/mouse/rat GDF8 R&D Systems Cat#788-G8 Recombinant human/mouse/rat GDF11 R&D Systems Cat#1958-GD Recombinant human BMP2 R&D Systems Cat#355-BM Recombinant human BMP4 R&D Systems Cat#314-BP Recombinant human BMP5 R&D Systems Cat#615-BMC Recombinant human BMP6 R&D Systems Cat#507-BP Recombinant human BMP7 R&D Systems Cat354-BP Recombinant human BMP9 R&D Systems Cat#3209-BP Recombinant human BMP10 R&D Systems Cat#2926-BP Recombinant mouse BMP13 R&D Systems Cat#855-G6 Regeneron - Internal Recombinant human BMP14 R&D Systems Cat#8340-G5 Recombinant human long-R3-IGF1 R&D System Cat#83350-GMP Recombinant human TNFa R&D Systems Cat#210-TA/CF Anti-ActRIIA/B monoclonal antibody Regeneron N/A MyoTonic Basal Medium Cook MyoSite Cat#MB-2222 MyoTonic Growth Supplement Cook MyoSite Cat#MS-3333 Serum-free MyoTonic differentiation medium Cook MyoSite Cat#MD-9999 Horse serum Gibco Cat#16050130 Penicillin-Streptomycin (10,000 U/mL) Gibco Cat#15140-122 0.025% Trypsin Gibco Cat#25300-054 Puromycin Gibco Cat#A11138-03 Goat serum Thermo Fisher Scientific Cat#500622 Triton-X 100 Sigma Aldrich Cat#93443 DAPI Biolegend Cat#422801 RIPA Lysis Buffer Thermo Fisher Scientific Cat#89900 HaltTM protease and phosphatase inhibitor Thermo Fisher Scientific Cat#78440 Immobilon® Block Chemiluminescent Blocker Millipore Cat#WBAVDCH01 PierceTM ECL Western Blotting Substrate Thermo Fisher Scientific Cat#32106 Immobilon Chemiluminescent HRP Substrate Millipore Cat#WBKLS0500 TBS with TweenTM (TBST) Thermo Fisher Scientific Cat#J77500-K8 Commercial Assays PierceTM BCA Protein Assay Kits Thermo Fisher Scientific Cat#23227 MagMax-96 total RNA isolation kit Thermo Fisher Scientific Cat#AM1830 SuperScript II VILO cDNA Synthesis kit Thermo Fisher Scientific Cat#11754250 .. Human Skeletal Muscle Derived Cells (SKMDC) Cook Myosite Cat#SK-1111; Lot# P01059-14M

    cDNA Synthesis:

    Article Title: ActRII or BMPR ligands inhibit skeletal myoblast differentiation, and BMPs promote heterotopic ossification in skeletal muscles in mice
    Article Snippet: Rabbit monoclonal anti-phospho-SMAD2 (Ser465/467) Cell Signaling Technology Cat#3108; RRID:AB_490941 Rabbit monoclonal phospho-Smad3 (Ser423/425) Cell Signaling Technology Cat#9520; RRID:AB_2193207 Rabbit monoclonal anti-SMAD2/3 Cell Signaling Technology Cat#8685; RRID:AB_10889933 Rabbit monoclonal anti-SMAD3 Cell Signaling Technology Cat#9523; RRID:AB_2193182 Rabbit monoclonal phospho-Smad1 (Ser206) Cell Signaling Technology Cat#5753; RRID:AB_10706171 Rabbit monoclonal phospho-Smad1/5 (Ser463/465) Cell Signaling Technology Cat#9516; RRID:AB_491015 Rabbit polyclonal anti-SMAD1 Cell Signaling Technology Cat#9743; RRID:AB_2107780 Rabbit polyclonal anti-SMAD5 Cell Signaling Technology Cat#9517; RRID:AB_2193632 Rabbit monoclonal anti-vinculin Cell Signaling Technology Cat#13901; RRID:AB_2728768 Rabbit monoclonal anti-GAPDH Sigma Aldrich Cat#G9295-200ul Peroxidase AffiniPureTM Goat Anti-Rabbit IgG Jackson Cat#111-035-003 Mouse monoclonal anti-Myosin Heavy Chain/MYHC antibody [clone A4.1025] Abcam Cat#ab37484; RRID:AB_2921304 Goat polyclonal anti-Mouse IgG, Alexa FluorTM Plus 488 Thermo Fisher Scientific Cat#A32723; RRID:AB_2633275 .. PBS Gibco Cat#14190-144 BSA Sigma Aldrich Cat#A0336 Glycerol invitrogen Cat#15514011 Recombinant human/mouse/rat Activin A R&D Systems Cat#338-AC Recombinant human/mouse/rat GDF8 R&D Systems Cat#788-G8 Recombinant human/mouse/rat GDF11 R&D Systems Cat#1958-GD Recombinant human BMP2 R&D Systems Cat#355-BM Recombinant human BMP4 R&D Systems Cat#314-BP Recombinant human BMP5 R&D Systems Cat#615-BMC Recombinant human BMP6 R&D Systems Cat#507-BP Recombinant human BMP7 R&D Systems Cat354-BP Recombinant human BMP9 R&D Systems Cat#3209-BP Recombinant human BMP10 R&D Systems Cat#2926-BP Recombinant mouse BMP13 R&D Systems Cat#855-G6 Regeneron - Internal Recombinant human BMP14 R&D Systems Cat#8340-G5 Recombinant human long-R3-IGF1 R&D System Cat#83350-GMP Recombinant human TNFa R&D Systems Cat#210-TA/CF Anti-ActRIIA/B monoclonal antibody Regeneron N/A MyoTonic Basal Medium Cook MyoSite Cat#MB-2222 MyoTonic Growth Supplement Cook MyoSite Cat#MS-3333 Serum-free MyoTonic differentiation medium Cook MyoSite Cat#MD-9999 Horse serum Gibco Cat#16050130 Penicillin-Streptomycin (10,000 U/mL) Gibco Cat#15140-122 0.025% Trypsin Gibco Cat#25300-054 Puromycin Gibco Cat#A11138-03 Goat serum Thermo Fisher Scientific Cat#500622 Triton-X 100 Sigma Aldrich Cat#93443 DAPI Biolegend Cat#422801 RIPA Lysis Buffer Thermo Fisher Scientific Cat#89900 HaltTM protease and phosphatase inhibitor Thermo Fisher Scientific Cat#78440 Immobilon® Block Chemiluminescent Blocker Millipore Cat#WBAVDCH01 PierceTM ECL Western Blotting Substrate Thermo Fisher Scientific Cat#32106 Immobilon Chemiluminescent HRP Substrate Millipore Cat#WBKLS0500 TBS with TweenTM (TBST) Thermo Fisher Scientific Cat#J77500-K8 Commercial Assays PierceTM BCA Protein Assay Kits Thermo Fisher Scientific Cat#23227 MagMax-96 total RNA isolation kit Thermo Fisher Scientific Cat#AM1830 SuperScript II VILO cDNA Synthesis kit Thermo Fisher Scientific Cat#11754250 .. Human Skeletal Muscle Derived Cells (SKMDC) Cook Myosite Cat#SK-1111; Lot# P01059-14M



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